Catalog Number : 500-P48
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hSCF. Anti-Human SCF specific antibody was purified by affinity chromatography employing immobilized hSCF matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human SCF (BioGems Catalog #300-07)
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 0.5 μg/ml - 1.0 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with enzyme induced antigen retrieval (proteinase K) at room temperature. Optimal concentrations and conditions may vary.
*Additional Immunostaining data available. Please contact Tech Support for information.
To yield one-half maximal inhibition [ND50] of the biological activity of hSCF (10.00 ng/ml), a concentration of 0.64-0.96 µg/ml of this antibody is required.
To detect hSCF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human SCF (60-291BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF.
To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.