Anti-Human sCD40 Ligand
Catalog Number : 500-P142G
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L. Anti-Human sCD40L specific antibody was purified by affinity chromatography employing immobilized hsCD40L matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human sCD40 Ligand (BioGems Catalog #310-02)
This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentrations are 2.5 μg/ml - 5.0 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. The recommended antigen retrieval is heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.
To detect hsCD40-Ligand by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human sCD40-Ligand (60-101) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40-Ligand.
To detect hsCD40-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40-Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.