Catalog Number : 500-P227
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCD22. Anti-Human CD22 specific antibody was purified by affinity chromatography employing immobilized hCD22 matrix.
|Application:||ELISA, Western Blot|
Anti-Human sCD22 (BioGems Catalog #100-01)
To detect hCD22 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human CD22 (60-096BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCD22.
To detect hCD22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCD22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.