Catalog Number : 500-P183G
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hResistin. Anti-Human Resistin specific antibody was purified by affinity chromatography employing immobilized hResistin matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human Resistin (BioGems Catalog #450-19)
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To detect hResistin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human Resistin (60-289BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hResistin.
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.