Catalog Number : 500-P217
Produced from sera of rabbits pre-immunized with highly pure recombinant Human RELMβ. Anti-Human RELMβ specific antibody was purified by affinity chromatography employing immobilized Human RELMβ matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human RELMβ (BioGems Catalog #450-22)
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.0 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.
To detect Human RELMβ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human RELMβ (60-288BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human RELMβ.
To detect Human RELMβ by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human RELMβ is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.