Catalog Number : 500-P05
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPF-4. Anti-Human PF-4 specific antibody was purified by affinity chromatography employing immobilized hPF-4 matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human PF-4 (BioGems Catalog #300-16)
This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1.0 mg/mL - 2.0 mg/mL with an overnight incubation at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
To detect hPF-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human PF-4 (60-272BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPF-4.
To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.