Anti-Human PF-4

Grouped product items
SizePrice Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00
Catalog Number : 500-P05

description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPF-4. Anti-Human PF-4 specific antibody was purified by affinity chromatography employing immobilized hPF-4 matrix.

Additional Information

Application:ELISA, Western Blot, Immunohistochemistry
Immunogen
Anti-Human PF-4 (BioGems Catalog #300-16)
Clone:
Polyclonal Rabbit
Format:
purified
Applications:
Immunohistochemistery:
This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1.0 mg/mL - 2.0 mg/mL with an overnight incubation at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
Sandwich ELISA:
To detect hPF-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human PF-4 (60-272BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPF-4.
Western Blot:
To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
Human
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