Catalog Number : 500-P46
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-AA. Anti-Human PDGF-AA specific antibody was purified by affinity chromatography employing immobilized hPDGF-AA matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human PDGF-AA (BioGems Catalog #100-13A)
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 μg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (5.0 ng/ml), a concentration of 0.13-0.20 µg/ml of this antibody is required.
To detect hPDGF-AA by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human PDGF-AA (60-265BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA.
To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.