Anti-Human PAI-1

Grouped product items
SizePrice Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00
Catalog Number : 500-P260

description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPAI-1 (human Plasminogen Activator Inhibitor-1). Anti-Human PAI-1 specific antibody was purified by affinity chromatography employing immobilized hPAI-1 matrix.

Additional Information

Application:ELISA, Western Blot, Neutralization, Immunohistochemistry
Immunogen
Anti-Human PAI-1 (BioGems Catalog #140-04)
Clone:
Polyclonal Rabbit
Format:
purified
Applications:
Immunohistochemistery:
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 62.5 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

*Additional Immunostaining data available. Please contact Tech Support for information.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hPAI-1 (4.5 μg/ml), a concentration of at least 15.0 μg/ml of this antibody is required.
Sandwich ELISA:
To detect hPAI-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human PAI-1 (60-263BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPAI-1.
Western Blot:
To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
Human
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