Anti-Human OPG
Catalog Number : 500-P149
description
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hOPG (human Osteoprotegerin). Anti-Human OPG specific antibody was purified by affinity chromatography employing immobilized hOPG matrix.
Additional Information
| Application: | ELISA, Western Blot, Immunohistochemistry |
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Immunogen
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Anti-Human OPG (BioGems Catalog #450-14)
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Clone:
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Polyclonal Rabbit
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Format:
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purified
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Applications:
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To detect Human OPG by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human OPG (500-P149Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human OPG.
To detect hOPG by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human OPG (60-257BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOPG.
To detect hOPG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOPG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Reactivity:
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Human
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