Anti-Human MIP-1β

Grouped product items
SizePrice Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00
Catalog Number : 500-P38B

description

Produced from sera of rabbits pre-immunized with highly pure recombinant Human MIP-1β. Anti-Human MIP-1β specific antibody was purified by affinity chromatography employing immobilized Human MIP-1β matrix.

Additional Information

Application:ELISA, Western Blot, Neutralization, Immunohistochemistry
Immunogen
Anti-Human MIP-1β (BioGems Catalog #300-09)
Clone:
Polyclonal Rabbit
Format:
purified
Applications:
Immunohistochemistery:
This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma.  The recommended concentration is 1.0 mg/ml with 2 hour incubation at room temperature.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary. 
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human MIP-1β (50.00 ng/ml), a concentration of 1.75-3.00 µg/ml of this antibody is required.
Sandwich ELISA:
To detect Human MIP-1β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human MIP-1β (60-225BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-1β.
Western Blot:
To detect Human MIP-1β by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
Human
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