Catalog Number : 500-P38B
Produced from sera of rabbits pre-immunized with highly pure recombinant Human MIP-1β. Anti-Human MIP-1β specific antibody was purified by affinity chromatography employing immobilized Human MIP-1β matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human MIP-1β (BioGems Catalog #300-09)
This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 1.0 mg/ml with 2 hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of Human MIP-1β (50.00 ng/ml), a concentration of 1.75-3.00 µg/ml of this antibody is required.
To detect Human MIP-1β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human MIP-1β (60-225BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-1β.
To detect Human MIP-1β by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.