Catalog Number : 500-P50
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIG. Anti-Human MIG specific antibody was purified by affinity chromatography employing immobilized hMIG matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human MIG (BioGems Catalog #300-26)
This antibody stained formalin-fixed paraffin-embedded sections of human renal tumor with parenchyma tissue. The recommended concentration is 0.25μg/mL- 0.50μg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate buffer. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.0 ng/ml), a concentration of 5.0-7.0 µg/ml of this antibody is required.
To detect hMIG by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human MIG (60-223BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIG.
To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.