Anti-Human Midkine

Grouped product items
Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00
Catalog Number : 500-P171

description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMidkine. Anti-Human Midkine specific antibody was purified by affinity chromatography employing immobilized hMidkine matrix.

Additional Information

Application: ELISA, Western Blot, Immunohistochemistry
Immunogen
Anti-Human Midkine (BioGems Catalog #450-16)
Clone:
Polyclonal Rabbit
Format:
purified
Applications:
Immunohistochemistery:
This antibody stained human brain stroke (including control cortex and stroke core areas) tissue.  The primary antibody was incubated at 2.5 mg/ml overnight at 4˚C.  This was followed by a fluorophore conjugated secondary antibody.  Optimal concentrations and conditions may vary.

Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.
Sandwich ELISA:
To detect hMidkine by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human Midkine (60-221BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine.
Western Blot:
To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
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