Catalog Number : 500-P35
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-2. Anti-Human MCP-2 specific antibody was purified by affinity chromatography employing immobilized hMCP-2 matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human MCP-2 (BioGems Catalog #300-15)
This antibody stained formalin-fixed, paraffin-embedded sections of human normal gastric mucosa. The recommended concentration is 0.75mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
To detect hMCP-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human MCP-2 (60-213BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-2.
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.