Catalog Number : 500-P34
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-1(MCAF). Anti-Human MCP-1(MCAF) specific antibody was purified by affinity chromatography employing immobilized hMCP-1(MCAF) matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human MCP-1 (BioGems Catalog #300-04)
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5 μg/ml and a two-hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
To detect hMCP-1(MCAF) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human MCP-1(MCAF) (60-212BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1(MCAF).
To detect hMCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.