Catalog Number : 500-P40
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLymphotactin. Anti-Human Lymphotactin specific antibody was purified by affinity chromatography employing immobilized hLymphotactin matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human Lymphotactin (BioGems Catalog #300-20)
This antibody stained formalin-fixed, paraffin-embedded sections of human normal kidney. The recommended concentration is 0.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of hLymphotactin (60 ng/ml), a concentration of 1.7-3.0 µg/ml of this antibody is required.
To detect hLymphotactin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human Lymphotactin (60-208BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLymphotactin.
To detect hLymphotactin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLymphotactin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.