Catalog Number : 500-P93
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIP-10. Anti-Human IP-10 specific antibody was purified by affinity chromatography employing immobilized hIP-10 matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human IP-10 (BioGems Catalog #300-12)
This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of Human IP-10 (100.0 ng/ml), a concentration of 5.0 – 8.0 μg/ml of this antibody is required.
To detect hIP-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IP-10 (60-196BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIP-10.
To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.