Catalog Number : 500-P22
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-2. Anti-Human IL-2 specific antibody was purified by affinity chromatography employing immobilized hIL-2 matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry. Neutralization|
Anti-Human IL-2 (BioGems Catalog #200-02)
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125μg/ml-0.250μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-2 (0.8 ng/ml), a concentration of 0.26 - 0.40 µg/ml of this antibody is required.
To detect hIL-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IL-2 (60-002BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-2.
To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.