Catalog Number : 500-P191
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-21. Anti-Human IL-21 specific antibody was purified by affinity chromatography employing immobilized hIL-21 matrix.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Human IL-21 (BioGems Catalog #200-21)
This antibody exhibits complete neutralization of the biological activity of hIL-21 in a human B cell stimulation assay.
To detect hIL-21 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IL-21 (60-021BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-21.
To detect hIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.