Catalog Number : 500-P209
Produced from sera of rabbits immunized with highly pure recombinant Human IL-1RA. Anti-Human IL-1RA specific antibody was purified by affinity chromatography employing an immobilized Human IL-1RA matrix.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Human IL-1RA (BioGems Catalog #200-01RA)
To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-1RA (50.0 ng/ml) and Human IL-1α(50.0 pg/ml), a concentration of 2.5-3.7 μg/ml of this antibody is required.
To detect Human IL-1RA by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IL-1RA (60-001RABT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-1RA.
To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.