Anti-Human IL-1RA

Grouped product items
SizePrice Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00
Catalog Number : 500-P209

description

Produced from sera of rabbits immunized with highly pure recombinant Human IL-1RA. Anti-Human IL-1RA specific antibody was purified by affinity chromatography employing an immobilized Human IL-1RA matrix.

Additional Information

Application:ELISA, Western Blot, Neutralization
Immunogen
Anti-Human IL-1RA (BioGems Catalog #200-01RA)
Clone:
Polyclonal Rabbit
Format:
purified
Applications:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-1RA (50.0 ng/ml) and Human IL-1α(50.0 pg/ml), a concentration of 2.5-3.7 μg/ml of this antibody is required.
Sandwich ELISA:
To detect Human IL-1RA by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IL-1RA (60-001RABT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-1RA.
Western Blot:
To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
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