Catalog Number : 500-P248
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17B. Anti-Human IL-17B specific antibody was purified by affinity chromatography employing immobilized hIL-17B matrix.
|Application:||ELISA, Western Blot|
Anti-Human IL-17B (BioGems Catalog #200-28)
To detect hIL-17B by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IL-17B (60-017BBT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17B.
To detect hIL-17B by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17B is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.