Catalog Number : 500-P20
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-10. Anti-Human IL-10 specific antibody was purified by affinity chromatography employing immobilized hIL-10 matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human IL-10 (BioGems Catalog #200-10)
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-10 (1.0 ng/ml), a concentration of 1.2-1.8 µg/ml of this antibody is required.
To detect hIL-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IL-10 (60-010BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-10.
To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.