Anti-Human IL-1α

Grouped product items
SizePrice Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00
Catalog Number : 500-P21A

description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-1α. Anti-Human IL-1α specific antibody was purified by affinity chromatography employing immobilized hIL-1α matrix.

Additional Information

Application:ELISA, Western Blot, Neutralization, Immunohistochemistry
Immunogen
Anti-Human IL-1α (BioGems Catalog #200-01A)
Clone:
Polyclonal Rabbit
Format:
purified
Applications:
Immunohistochemistery:
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 μg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen. Enzyme induced antigen retrieval was performed with proteinase K. Optimal concentrations and conditions may vary.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1α (20 ng/ml), a concentration of 1.0 - 2.0 µg/ml of this antibody is required.
Sandwich ELISA:
To detect hIL-1α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IL-1α (60-001ABT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-1α.
Western Blot:
To detect hIL-1α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity:
© BioGems International, Inc. All rights reserved.