Catalog Number : 500-P230
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP3. Anti-Human IGF-BP3 specific antibody was purified by affinity chromatography employing immobilized hIGF-BP3 matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human IGF-BP3 (BioGems Catalog #100-08)
This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentrations are 2.5µg/ml-0.5 µg/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.
To detect hIGF-BP3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IGF-BP3 (60-190BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3.
To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.