Catalog Number : 500-P247
Produced from sera of rabbits pre-immunized with highly pure recombinant Human IFN-λ2. Anti-Human IFN-λ2 specific antibody was purified by affinity chromatography employing immobilized Human IFN-λ2 matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human IFN-λ2 (BioGems Catalog #300-02K)
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 mg/ml overnight at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen and proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
To detect Human IFN-λ2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IFN-λ2 (60-184BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-λ2.
To detect Human IFN-λ2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-λ2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.