Catalog Number : 500-P287
Produced from sera of rabbits immunized with highly pure recombinant Human ICAM-1. Anti-Human ICAM-1 specific antibody was purified by affinity chromatography employing an immobilized Human ICAM-1 matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human ICAM-1 (BioGems Catalog #150-05)
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal adenocarcinoma (moderately differentiated). The recommended concentration is 0.25 mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of Human ICAM-1 (10 ng/ml), a concentration of 8.0 μ/ml of this antibody is required.
To detect Human ICAM-1 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human ICAM-1 (60-180BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human ICAM-1.
To detect Human ICAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human ICAM-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.