Catalog Number : 500-P33
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGM-CSF. Anti-Human GM-CSF specific antibody was purified by affinity chromatography employing immobilized hGM-CSF matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human GM-CSF (BioGems Catalog #300-03)
This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.25 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of hGM-CSF (1.0 ng/ml), a concentration of 0.13-0.20 µg/ml of this antibody is required.
To detect hGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human GM-CSF (60-167BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGM-CSF.
To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.