Anti-Human Fractalkine

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Catalog Number : 500-P98


Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFractalkine. Anti-Human Fractalkine specific antibody was purified by affinity chromatography employing immobilized hFractalkine matrix.

Additional Information

Application: ELISA, Western Blot, Immunohistochemistry
Anti-Human Fractalkine (BioGems Catalog #300-31)
Polyclonal Rabbit
This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas malignant ductal adenocarcinoma. The recommended concentration is 0.25µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

*Additional Immunostaining data available. Please contact Tech Support for information.
Sandwich ELISA:
To detect hFractalkine by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human Fractalkine (60-149BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFractalkine.
Western Blot:
To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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