Catalog Number : 500-P207
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFollistatin. Anti-Human Follistatin specific antibody was purified by affinity chromatography employing immobilized hFollistatin matrix.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Human Follistatin (BioGems Catalog #120-13)
To yield one-half maximal inhibition [ND50] of the biological activity of Human Follistatin (100.0 ng/ml), a concentration of 8.0 - 12.0 μg/ml of this antibody is required.
To detect Human Follistatin by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human Follistatin (60-148BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Follistatin.
To detect Human Follistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Follistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.