Anti-Human FGF-acidic

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Catalog Number : 500-P17


Produced from sera of rabbits pre-immunized with highly pure recombinant Human FGF-acidic. Anti-Human FGF-acidic specific antibody was purified by affinity chromatography employing immobilized Human FGF-acidic matrix.

Additional Information

Application: ELISA, Western Blot, Neutralization, Immunohistochemistry
Anti-Human FGF-acidic (BioGems Catalog #100-17A)
Polyclonal Rabbit
This antibody stained formalin-fixed, paraffin-embedded sections of human normal kidney.  The recommended concentration is 0.050 µg/mL-0.125 µg/mL with an overnight incubation at 4°C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary. 
To yield one-half maximal inhibition [ND50] of the biological activity of Human FGF-acidic (10.0 ng/ml), a concentration of 1.0-2.5 µg/ml of this antibody is required.
Sandwich ELISA:
To detect Human FGF-acidic by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human FGF-acidic (60-132BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human FGF-acidic.
Western Blot:
To detect Human FGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human FGF-acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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