Catalog Number : 500-P158
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-4. Anti-Human FGF-4 specific antibody was purified by affinity chromatography employing immobilized hFGF-4 matrix.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human FGF-4 (BioGems Catalog #100-31)
This antibody stained formalin-fixed paraffin-embedded sections of normal breast tissue from a breast carcinoma. The recommended concentration is 0.25 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
To detect hFGF-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human FGF-4 (60-134BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-4.
To detect hFGF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.