Catalog Number : 500-P262
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEndostatin (human Endostatin). Anti-Human Endostatin specific antibody was purified by affinity chromatography employing immobilized hEndostatin matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human Endostatin (BioGems Catalog #150-01)
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 μg/ml-0.250 μg/ml with an overnight incubation at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To yield one-half the maximal inhibition [ND50] of the biological activity of Human Endostatin (0.5 μg/ml), a concentration of 2.0 μg/ml of this antibody is required.
To detect hEndostatin by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human Endostatin (60-120BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEndostatin.
To detect hEndostatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEndostatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.