Anti-Human EGF Receptor
Catalog Number : 500-P306
Produced from sera of rabbits immunized with highly pure recombinant Human EGF Receptor. Anti-Human EGF Receptor specific antibody was purified by affinity chromatography employing an immobilized Human EGF Receptor matrix.
|Application:||ELISA, Western Blot|
Anti-Human EGF Receptor (BioGems Catalog #100-15R)
To detect Human EGF Receptor by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human EGF Receptor (60-116BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human EGF Receptor.
To detect Human EGF Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human EGF Receptor is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.