Anti-Human CTGF
Catalog Number : 500-P252
description
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCTGF. Anti-Human CTGF specific antibody was purified by affinity chromatography employing immobilized hCTGF matrix.
Additional Information
| Application: | ELISA, Western Blot, Neutralization, Immunohistochemistry |
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Immunogen
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Anti-Human CTGF (BioGems Catalog #120-19)
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Clone:
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Polyclonal Rabbit
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Format:
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purified
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Applications:
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This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive malignant carcinoma. The recommended concentration is 0.125 μg/mL-0.250 μg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of hCTGF (5.0 μg/ml), a concentration of 6.0 - 9.0 μg/ml of this antibody is required.
To detect hCTGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human CTGF (60-107BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF.
To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Reactivity:
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