Catalog Number : 500-P254
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBetacellulin. Anti-Human Betacellulin specific antibody was purified by affinity chromatography employing immobilized hBetacellulin matrix.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human Betacellulin (BioGems Catalog #100-50)
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 2.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of Human Betacellulin (0.1 ng/ml), a concentration of ≤ 0.1 μg/ml of this antibody is required.
To detect hBetacellulin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human Betacellulin (60-081BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.
To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.