Catalog Number : 500-P161G
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hBD-2. Anti-Human BD-2 specific antibody was purified by affinity chromatography employing immobilized hBD-2 matrix.
|Application:||ELISA, Western Blot, Neutralization|
Anti-Human BD-2 (BioGems Catalog #300-49)
To yield one-half maximal inhibition [ND50] of the biological activity of Human BD-2 (1.0 μg/ml), a concentration of 5.0 - 8.0 mg/ml of this antibody is required.
To detect hBD-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human BD-2 (60-076BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-2.
To detect hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.