Animal-Free Recombinant Human Oncostatin-M (209 a.a.)
Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activate T cells, monocytes and Kaposi’s sarcoma cells, OSH can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi’s sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptor in hepatoma cells. OSM share several structural and functional characteristics with LIF, IL-6, and CNTF. Human OSM is active on murine cells. The human OSM gene encodes for a 252 amino acid polypeptide, containing 25 amino acid signal sequence for secretion and a 227 precursor protein. Proteolytic processing of this precursor removes an 18 amino acid C-terminal peptide and generates the mature OSM form. Recombinant human Oncostatin M is a 23.6 kDa protein, containing 209 amino acid residues. Manufactured using all non-animal reagents.
Above 98% as determined by SDS-PAGE Analysis.
The ED50as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
AAIGSCSKE YRVLLGQLQ KQTDLMQDT SRLLDPYIR IQGLDVPKL REHCRERPG AFPSEETLR GLGRRGFLQ TLNATLGCV LHRLADLEQ RLPKAQDLE RSGLNIEDL EKLQMARPN ILGLRNNIY CMAQLLDNS DTAEPTKAG RGASQPPTP TPASDAFQR KLEGCRFLH GYHRFMHSV GRVFSKWGE SPNRSRRHS PHQALRKGV RR
|Research Interest:||Innate Immunity, Stem Cell Research, Immunology, Angiogenesis, Bone Biology, Inflammation|
|Cell Type:||Macrophage / Monocyte, T Cells, Dendritic Cells|
|Protein Cross Reactivity:||NULL|