Animal-Free Recombinant Human LIF
LIF is a pleiotrophic factor produced by multiple cell types including T cells, myelomoncytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant human LIF is a 19.7 kDa protein containing 180 amino acids residues including three disulfide bonds. Manufactured using all non-animal reagents.
Above 98% as determined by SDS-PAGE Analysis.
Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
SPLPITPVN ATCAIRHPC HNNLMNQIR SQLAQLNGS ANALFILYY TAQGEPFPN NLDKLCGPN VTDFPPFHA NGTEKAKLV ELYRIVVYL GTSLGNITR DQKILNPSA LSLHSKLNA TADILRGLL SNVLCRLCS KYHVGHVDV TYGPDTSGK DVFQKKKLG CQLLGKYKQ IIAVLAQAF
|Research Interest:||Stem Cell Research, Immunology, Proliferation and Cell Cycle, Metabolic Regulation and Diabetes|
|Cell Type:||T Cells, Stem Cells|
|Protein Cross Reactivity:||Mouse|