Animal-Free Recombinant Human BDNF
description
BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0 kDa homodimer of two 120 amino acid subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical. Manufactured using all non-animal reagents.
Additional Information
Source: | E. Coli |
Purity: | Above 98% as determined by SDS-PAGE Analysis. |
Cross Reactivity: | Chicken, Frog, Guinea Pig, Hamster, Monkey, Mouse, Rat |
Biological Activity | Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1.0 μg/ml. |
AA Sequence | MHSDPARRG ELSVCDSIS EWVTAADKK TAVDMSGGT VTVLEKVPV SKGQLKQYF YETKCNPMG YTKEGCRGI DKRHWNSQC RTTQSYVRA LTMDSKKRI GWRFIRIDT SCVCTLTIK RGR |
Research Interest: | Stem Cell Research, Neurosciences, Proliferation and Cell Cycle |
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Cell Type: | Neurons |
Application: | FA |
Protein Cross Reactivity: | Chicken, Frog, Guinea Pig, Hamster, Monkey, Mouse, Rat |