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Recombinant Murine WNT-3a



Catalog Number : 315-20

Description

Wnt-3a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. Expression of Wnt-3a occurs primarily along the dorsal midline across overlapping regions of the Central Nervous System (CNS). Wnt-3a signaling is essential for various morphogenetic events including embryonic patterning, cell determination, cell proliferation, CNS development, and cytoskeletal formation. Like other members of this family, Wnt-3a contains a highly conserved lipid modified cysteine rich domain that is essential for cell signaling. During a biochemical process called the canonical Wnt pathway; Wnt family members bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of β-Catenein degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors to promote gene expression. Lack of Wnt signaling disrupts transcriptional activation of tumor suppressor genes and has shown to result in neoplastic transformation, oncogenesis, and human degenerative diseases. Recombinant murine Wnt-3a is a monomeric glycoprotein containing 334 amino acid residues. Due to glycosylation, the murine Wnt-3a migrates at an apparent molecular weight of approximately 38.0-41.0 kDa by SDS-PAGE analysis under non-reducing conditions.

Additional Information

Source:
Cell Culture
 
Purity:
Above 75% as determined by SDS-PAGE Analysis.
 
Cross Reactivity:
NULL
 
Biological Activity
The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells. The expected ED50for this effect is 0.4-0.6 ng/ml.
 
AA Sequence
SYPIWWSLA VGPQYSSLS TQPILCASI PGLVPKQLR FCRNYVEIM PSVAEGVKA GIQECQHQF RGRRWNCTT VSNSLAIFG PVLDKATRE SAFVHAIAS AGVAFAVTR SCAEGSAAI CGCSSRLQG SPGEGWKWG GCSEDIEFG GMVSREFAD ARENRPDAR SAMNRHNNE AGRQAIASH MHLKCKCHG LSGSCEVKT CWWSQPDFR TIGDFLKDK YDSASEMVV EKHRESRGW VETLRPRYT YFKVPTERD LVYYEASPN FCEPNPETG SFGTRDRTC NVSSHGIDG CDLLCCGRG HNARTERRR EKCHCVFHW CCYVSCQEC TRVYDVHTC K
Research Interest: Stem Cell Research, Cancer, Bone Biology, Proliferation and Cell Cycle
Protein Cross Reactivity: NULL

Availability: In stock

Size Price Qty
2µg
$80.00
10µg
$195.00
100µg
$1,900.00
500µg
$5,880.00
1mg
$9,800.00