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Recombinant Human WNT-7A



Catalog Number : 120-31

Description

Wnt-7a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. It is expressed in placenta, kidney, testis, uterus, fetal lung and fetal and adult brain. Most Wnt proteins can signal though a mechanism called the canonical Wnt pathway, in which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of β-Catenein degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors and induces the expression of numerous genes. Increased Wnt/β-Catenein signaling is associated with tumorigenesis in a diverse set of human cancers. However, Wnt-7a/Frizzled-9 signaling has been shown to act as a tumor suppressor in non-small cell lung cancers. Recombinant human Wnt-7a is a 35.5 kDa glycoprotein containing 318 amino acids. Due to glycosylation, Wnt-7a migrates between 40-55 kDa by SDS-PAGE gel under unreduced conditions

Additional Information

Source:
HEK293 cells
 
Purity:
Above 80% as determined by SDS-PAGE Analysis.
 
Cross Reactivity:
NULL
 
Biological Activity
Determined by its ability to inhibit Wnt3a induced alkaline phosphatase production in MC3T3-E1 cells. The expected ED50 for this effect is 40-60 ng/ml.
 
AA Sequence
LGASIICNK IPGLAPRQR AICQSRPDA IIVIGEGSQ MGLDECQFQ FRNGRWNCS ALGERTVFG KELKVGSRE AAFTYAIIA AGVAHAITA ACTQGNLSD CGCDKEKQG QYHRDEGWK WGGCSADIR YGIGFAKVF VDAREIKQN ARTLMNLHN NEAGRKILE ENMKLECKC HGVSGSCTT KTCWTTLPQ FRELGYVLK DKYNEAVHV EPVRASRNK RPTFLKIKK PLSYRKPMD TDLVYIEKS PNYCEEDPV TGSVGTQGR ACNKTAPQA SGCDLMCCG RGYNTHQYA RVWQCNCKF HWCCYVKCN TCSERTEMY TCK
Research Interest: Stem Cell Research, Cancer, Inflammation, Proliferation and Cell Cycle
Cell Type: Stem Cells
Protein Cross Reactivity: NULL

Availability: In stock

Size Price Qty
3µg
$80.00
15µg
$195.00