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Recombinant Human NOGGIN



Catalog Number : 120-10C

Description

Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors. The interplay between TGF-β ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant human Noggin is a 46 kDa disulfide-linked homodimer (120-10C) consisting of two 206 amino acid polypeptide chains. Monomeric glycosylated Noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS PAGE analysis under reducing conditions.

Additional Information

Source:
HEK293 cells
 
Purity:
Above 95% as determined by SDS-PAGE Analysis.
 
Cross Reactivity:
Chicken, Mouse
 
Biological Activity
Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.
 
AA Sequence
QHYLHIRPA PSDNLPLVD LIEHPDPIF DPKEKDLNE TLLRSLLGG HYDPGFMAT SPPEDRPGG GGGAAGGAE DLAELDQLL RQRPSGAMP SEIKGLEFS EGLAQGKKQ RLSKKLRRK LQMWLWSQT FCPVLYAWN DLGSRFWPR YVKVGSCFS KRSCSVPEG MVCKPSKSV HLTVLRWRC QRRGGQRCG WIPIQYPII SECKCSC
Research Interest: Stem Cell Research, Bone Biology
Cell Type: Stem Cells
Protein Cross Reactivity: Chicken, Mouse

Availability: In stock

Size Price Qty
5µg
$80.00
20µg
$195.00
100µg
$780.00
500µg
$2,340.00
1mg
$3,900.00