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Recombinant Human LIF



Catalog Number : 300-05

Description

LIF is a pleiotrophic factor produced by multiple cell types including T cells, myelomoncytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant human LIF is a 19.7 kDa protein containing 180 amino acids residues including three disulfide bonds.

Additional Information

Source:
E. Coli
 
Purity:
Above 98% as determined by SDS-PAGE Analysis.
 
Cross Reactivity:
Mouse
 
Biological Activity
Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
 
AA Sequence
SPLPITPVN ATCAIRHPC HNNLMNQIR SQLAQLNGS ANALFILYY TAQGEPFPN NLDKLCGPN VTDFPPFHA NGTEKAKLV ELYRIVVYL GTSLGNITR DQKILNPSA LSLHSKLNA TADILRGLL SNVLCRLCS KYHVGHVDV TYGPDTSGK DVFQKKKLG CQLLGKYKQ IIAVLAQAF
Research Interest: Stem Cell Research, Immunology, Proliferation and Cell Cycle, Metabolic Regulation and Diabetes
Cell Type: T Cells, Stem Cells
Protein Cross Reactivity: Mouse

Availability: In stock

Size Price Qty
5µg
$80.00
25µg
$195.00
100µg
$700.00
500µg
$1,680.00
1mg
$2,800.00