Anti-Mouse CD28 Purified
description
The 37.51 monoclonal antibody specifically reacts with mouse CD28 molecule, a 45 kDa homodimer. It is expressed on most thymocytes, at low density on most of the CD4+ and CD8+ peripheral T lymphocytes, and on the natural killer cells. It is additionally found on activated splenocytes, mast cells, and thymocytes. CD 28 is a costimulator of T lymphocytes and a ligand for CD80 (B7-1), and CD86 (B7-2). The CD28 signaling stimulates the expression of IL-2 and IL-2 receptor and the cytotoxicity of CD3-activated T lymphocytes.
The 37.51 antibody stimulates the proliferation of T lymphocytes and natural killer cells and their respective cytokine production.
Additional Information
Clone:
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37.51
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Format:
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Purified
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Applications:
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FC, FA, IP
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Reactivity:
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Mouse
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Isotype:
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Golden Syrian Hamster IgG
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Research Interest: | Adaptive Immunity |
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Cell Type: | T Cells |
Application: | FC, FA, IP |
Clone: | 37.51 |
Preparation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Nandi, D., Gross, J. A., & Allison, J. P. (1994). CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells. The Journal of Immunology, 152(7), 3361-3369. Gross, J. A., Callas, E. L. E. N. I., & Allison, J. P. (1992). Identification and distribution of the costimulatory receptor CD28 in the mouse. The Journal of Immunology, 149(2), 380-388. Grossi, J. A., Raulet, D. H., & Allison, J. P. (1992). CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones.Nature, 356, 607-9. Nishio, M., Spielman, J., Lee, R. K., Nelson, D. L., & Podack, E. R. (1996). CD80 (B7. 1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis. The Journal of Immunology,157(10), 4347-4353. |