Anti-Mouse CD16 / CD32 Purified
The 2.4G2 monoclonal antibody specifically reacts with an epitope on the extracellular domain of the mouse CD16 (Fc γ III) and CD 32 (Fc γ II). CD16 and CD32 are low affinity receptors for the IgG Fc domain and are expressed by B lymphocytes, NK cells, kupffer cells, mast cells, monocytes, macrophages, granulocytes, immature thymocytes, neutrophils, and some activated mature T cells.
The 2.4G2 antibody blocks the binding of immunoglobulins to CD16 and CD32, and possibly to Fc γ I receptor.
FC, FA, IF, IP
|Research Interest:||Innate Immunity|
|Cell Type:||NK and NKT Cells, Macrophage / Monocyte|
|Application:||FC, FA, IF, IP|
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Araujo-Jorge, T. A. N. I. A., Rivera, M. T., el Bouhdidi, A. Y. A. C. H. I., Daëron, M. A. R. C., & Carlier, Y. (1993). An Fc gamma RII-, Fc gamma RIII-specific monoclonal antibody (2.4 G2) decreases acute Trypanosoma cruzi infection in mice. Infection and immunity, 61(11), 4925-4928.
Jensen, W. A., Marschner, S., Ott, V. L., & Cambier, J. C. (2001). FcgammaRIIB-mediated inhibition of T-cell receptor signal transduction involves the phosphorylation of SH2-containing inositol 5-phosphatase (SHIP), dephosphorylation of the linker of activated T-cells (LAT) and inhibition of calcium mobilization. Biochemical Society Transactions, 29(Pt 6), 840-846.
Vremec, D., Zorbas, M., Scollay, R., Saunders, D. J., Ardavin, C. F., Wu, L., & Shortman, K. (1992). The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells. The Journal of experimental medicine, 176(1), 47-58.