CFSE has been tested by flow cytometric analysis of stimulated human peripheral blood mononuclear cells. It can be used at concentrations ranging from 0.5 to 20 uM for cell labeling. For optimal performance, it is recommended that the used concentration be empirically determined for the assay of interest.
Ingulli, E. (2007). Tracing tolerance and immunity in vivo by CFSE-labeling of administered cells. In Immunological Tolerance (pp. 365-376). Humana Press.
Lyons, A. B., & Parish, C. R. (1994). Determination of lymphocyte division by flow cytometry. Journal of immunological methods, 171(1), 131-137.
Parish, C. R., Glidden, M. H., Quah, B. J., & Warren, H. S. (2009). Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Current protocols in immunology, 4-9.