Anti-Mouse Ly-6G (Gr-1) FITC

Grouped product items
Size Price Qty
25 μg
$35.00
100 μg
$70.00
500 μg
$205.00

Additional RB6-8C5 Formats

Cat.No. Format Starting From
83122-60 PE $ 45.00
83122-77 PE-Cyanine7 $ 70.00
83122-20 Purified $ 70.00
83122-25 SAFIRE Purified $ 135.00
83122-50 FITC $ 35.00
83122-30 Biotin $ 30.00
83122-80 APC $ 50.00
83122-40 BG Violet 450 $ 80.00
Catalog Number : 83122-50

description

The RB6-8C5 monoclonal antibody reacts with the mouse Ly-6G (also known as Gr-1). The Ly-6G protein is a myeloid differentiation antigen of 21-25 kDa, expressed in a regulated manner by the myeloid lineage in the bone marrow, where the level of antigen expression is correlated with the granulocyte maturation and differentiation. In the bone marrow, the antigen is not expressed by the erythroid cells.

From the peripheric cells, RB6-8C5 binds with monocytes, neutrophils and eosinophils.

As a marker for the mouse monocytes, macrophages and granulocytes, the RB6-8C5 antibody is usually combined with M1/70, a macrophage labeling antibody (Anti-CD11b), for phenotypic analysis.

product image
C57BI/6 bone marrow cells were stained with FITC RB6-8C5 with relevant isotype control in Red.

Additional Information

Clone:
RB6-8C5
Format:
FITC
Applications:
FC
Reactivity:
Mouse
Isotype:
Rat IgG2b, kappa
Research Interest: Innate Immunity
Cell Type: Macrophage / Monocyte
Application: FC
Clone: RB6-8C5
Preparation:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:

Fleming, T. J., Fleming, M. L., & Malek, T. R. (1993). Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.The Journal of Immunology151(5), 2399-2408.

Hestdal, K., Ruscetti, F. W., Ihle, J. N., Jacobsen, S. E., Dubois, C. M., Kopp, W. C., ... & Keller, J. R. (1991). Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. The Journal of Immunology,147(1), 22-28.

Huang, B., Zhao, J., Shen, S., Li, H., He, K. L., Shen, G. X., ... & Feng, Z. H. (2007). Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Research67(9), 4346-4352.

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