Anti-Mouse Ly-6G BG Violet 500

Grouped product items
Size Price Qty
25 ug
$85.00
100 ug
$220.00

Additional 1A8 Formats

Cat.No. Format Starting From
83112-87 APC-Cyanine7 $ 80.00
83112-50 FITC $ 60.00
83112-80 APC $ 70.00
83112-70 PerCP-Cyanine5.5 $ 85.00
83112-40 BG Violet 450 $ 85.00
83112-25 SAFIRE Purified $ 135.00
83112-60 PE $ 50.00
83112-77 PE-Cyanine7 $ 80.00
83112-45 BG Violet 500 $ 85.00
Catalog Number : 83112-45

description

The 1A8 monoclonal antibody specifically reacts with the 21-25 kDa glycophosphatidylinositol-anchored protein known as Ly-6G, expressed by the granulocytes from the bone marrow and periphery neutrophils. Ly-6G and Ly-6C form the Granulocyte Receptor-1 antigen (GR-1).

The binding of the 1A8 antibody to the Ly-6G can be blocked by another antibody, RB6-8C5, which also recognizes Ly-6C. While 1A8 is specific for only Ly-6G, RB6-8C5 also binds to Ly-6C.

BG Violet 500 conjugate is an alternative to the Pacific Orange and BD Horizon V500 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 500nm.

Additional Information

Clone:
1A8
Format:
BG Violet 500
Applications:
FC
Reactivity:
Mouse
Isotype:
Rat IgG2a, kappa
Research Interest: Adaptive Immunity
Cell Type: T Cells, NK and NKT Cells, Macrophage / Monocyte
Application: FC
Clone: 1A8
Preparation:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:

Fleming, T. J., Fleming, M. L., & Malek, T. R. (1993). Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.The Journal of Immunology151(5), 2399-2408.

Daley, J. M., Thomay, A. A., Connolly, M. D., Reichner, J. S., & Albina, J. E. (2008). Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. Journal of leukocyte biology83(1), 64-70.

Dietlin, T. A., Hofman, F. M., Lund, B. T., Gilmore, W., Stohlman, S. A., & van der Veen, R. C. (2007). Mycobacteria-induced Gr-1+ subsets from distinct myeloid lineages have opposite effects on T cell expansion. Journal of leukocyte biology81(5), 1205-1212.

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