Anti-Mouse CD4 BG Violet 450
Additional RM4-5 Formats
| Cat.No. | Format | Starting From |
|---|---|---|
| 06122-60 | PE | $ 30.00 |
| 06122-70 | PerCP-Cyanine5.5 | $ 80.00 |
| 06122-77 | PE-Cyanine7 | $ 70.00 |
| 06122-40 | BG Violet 450 | $ 75.00 |
| 06122-50 | FITC | $ 30.00 |
| 06122-87 | APC-Cyanine7 | $ 65.00 |
| 06122-80 | APC | $ 65.00 |
| 06122-45 | BG Violet 500 | $ 85.00 |
description
The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells. CD4 binds to the major histocompatibility complex class II (MHC class II) and enhances T lymphocyte development and mature T cells functions. In T lymphocytes, CD4 binds to the cytoplasmic tail of enzyme tyrosine kinase (p56lck).
Binding of RM4-5 is blocked by the anti-mouse CD4 clone GK1.5.
BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.
Additional Information
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Clone:
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RM4-5
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Format:
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BG Violet 450
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Applications:
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FC
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Reactivity:
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Mouse
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Isotype:
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Rat IgG2a, kappa
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| Research Interest: | Adaptive Immunity |
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| Cell Type: | Macrophage / Monocyte, T Cells, T Regulatory Cells, Th17 cells |
| Application: | FC |
| Clone: | RM4-5 |
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Preparation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Shevach, E. M. (2000). Regulatory T cells in autoimmmunity*. Annual review of immunology, 18(1), 423-449. Wu, L., Scollay, R., Egerton, M., Pearse, M., Spangrude, G. J., & Shortman, K. (1991). CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus. Bliss, S. K., Bliss, S. P., Beiting, D. P., Alcaraz, A., & Appleton, J. A. (2007). IL-10 regulates movement of intestinally derived CD4+ T cells to the liver. The Journal of Immunology, 178(12), 7974-7983. |