Anti-Human/Mouse CD11b APC
Additional M1/70 Formats
|03221-40||BG Violet 450||$ 110.00|
|03221-25||SAFIRE Purified||$ 135.00|
The M1/70 monoclonal antibody specifically reacts with the 170 kDa αM integrin chain of mouse CD11b from the Mac-1 integrin (CD11b/CD18). Mac-1 binds to C3bi, CD54 (ICAM-1), and fibrinogen, and it is expressed by granulocytes, macrophages, NK cells, myeloid-derived dendritic cells, microglia, activated lymphocytes, and mouse B-1 cells. The expression is up-regulated on activated neutrophils at the same time that L-selectin is shed from the cell surface. The M1/70 antibody is used for the detection of monocytes, granulocytes, and a subset of natural killer cells in human peripheral blood.
M1/70 blocks C3bi binding and cell adherence, but not cell-mediated lysis and it cross-reacts with human CD11b.
Rat IgG2b, kappa
|Research Interest:||Innate Immunity, Angiogenesis|
|Cell Type:||Macrophage / Monocyte, NK and NKT Cells|
|Protein Cross Reactivity:||Chimpanzee, Baboon, Cynomolgus, Rhesus|
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Sanchez-Madrid, F., Simon, P., Thompson, S., & Springer, T. A. (1983). Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1.The Journal of experimental medicine, 158(2), 586-602.
Ault, K. A., & Springer, T. A. (1981). Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. The Journal of Immunology, 126(1), 359-364.
Springer, T., Galfre, G., Secher, D. S., & Milstein, C. (1978). Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. European journal of immunology, 8(8), 539-551.