Anti-Human CD3 FITC

Grouped product items
Size Price Qty
25 tests
100 tests

Additional Hit3a Formats

Cat.No. Format Starting From
05111-50 FITC $ 40.00
05111-60 PE $ 50.00
05111-20 Purified $ 80.00
05111-80 APC $ 65.00
Catalog Number : 05111-50


The Hit3a monoclonal antibody specifically reacts with the ε chain of the CD3/T lymphocyte antigen receptor complex. The CD3 complex contains γ, δ, and ε chains, and it is part of the TCR complex, expressed by all mature T lymphocytes and by the thymocyte lineage. CD3 enhances the antigen recognition by signal transduction.

The HIT3a antibody cross-links with the TCR complex, initiating the cellular activation and proliferation, but it cannot be used for intracellular CD3 staining, as it is able to stain only the surface CD3 complex.

product image
Human peripheral blood lymphocytes were stained with FITC Hit3a with relevant isotype control in Red.

Additional Information

Mouse IgG2a, kappa
Research Interest: Adaptive Immunity
Cell Type: NK and NKT Cells, T Cells, T Regulatory Cells, Th17 cells
Application: FC
Clone: Hit3a
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

McMichael, A. J. (1987). Leucocyte typing III. Oxford University Press, Oxford. Norton AJ, Isaacson PG (1985)

Knapp W (1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989.

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York

Lanier, L. L., Allison, J. P., & Phillips, J. H. (1986). Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. The Journal of Immunology137(8), 2501-2507.

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